If the recovery is different, then components in the sample matrix are interfering with the analyte detection.What if a spike recovery experiment indicated that the sample matrix is affecting the results?
We recommend producing the standard curve using standard diluted in the sample matrix.
Any effects on the results from the sample matrix will also be present in the standard, and therefore comparison between the standard curve and the samples is more accurate.
Many of our ELISA kits contain a standard serum diluent for this purpose. For example, if neat biological sample is used, try diluting this in standard diluent.
Concentration of target protein in the sample To determine the concentration of target protein concentration in each sample, first find the mean absorbance value of the sample.
For these samples, the concentration obtained from the standard curve when analyzing the results must be multiplied by the dilution factor.This is expressed as a percentage of variance to the mean and indicates any inconsistencies and inaccuracies in the results.
Larger variance indicates greater inconsistency and error.
Narrative research can be considered both a research method in itself but also the phenomenon under study.
Narrative methods can be considered “real world measures” that are appropriate when “real life problems” are investigated.
From the Y axis of the standard curve graph, extend a horizontal line from this absorbance value to the standard curve.